The Baltic Sea Fluorescence Experiment (BASFE) was conducted between March 1 and 10, 1994 aboard the German research vessel Alexander von Humboldt as a collaborative project between the water research agency Instituto di Ricerca sulle Acque (IRSA) in Italy and the Institute for Baltic Sea Research (IOW) in Germany.
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Water was collected along several transects in the Baltic Sea and at anchor stations over the diel cycle from four or five depths using a rosette. A pulse amplitude modulated (PAM) fluorometer was used on sample concentrated by gentle filtration to measure phytoplankton photosynthesis.