Fuel for the Krebs cycle comes from lipids (fats) and carbohydrates, which both produce the molecule acetyl coenzyme-A (acetyl-CoA). This acetyl-CoA reacts in the first step of the eight step sequence of reactions that comprise the Krebs cycle, all of which occur inside mitochondria of eukaryotic cells. While the Krebs cycle does produce carbon dioxide, this cycle does not produce significant chemical energy in the form of adenosine triphosphate (ATP) directly, and this reaction sequence does not require any oxygen. Instead, this cycle produces NADH and FADH2, which feed into the respiratory cycle, also located inside of the mitochondria. It is the respiratory cycle that is responsible for production of large quantities of ATP and consumption of oxygen. In addition, the respiratory cycle converts NADH and FADH2 into reactants that the Krebs cycle requires to function. Thus, if oxygen is not present, the respiratory cycle cannot function, which shuts down the Krebs cycle. For this reason, the Krebs cycle is considered an aerobic pathway for energy production.
Clarke Earley (Lead Author);Lori Zaikowski (Topic Editor) "Krebs cycle". In: Encyclopedia of Earth. Eds. Cutler J. Cleveland (Washington, D.C.: Environmental Information Coalition, National Council for Science and the Environment). [First published in the Encyclopedia of Earth June 12, 2006; Last revised Date January 20, 2011; Retrieved June 19, 2013 <http://www.eoearth.org/article/Krebs_cycle?topic=49553>